Recombination between temperature-sensitive mutants of the arenavirus Pichinde.
نویسندگان
چکیده
High-frequency recombination was obtained with temperature-sensitive, conditionally lethal mutants of the arenavirus Pichinde.
منابع مشابه
In vitro and in vivo characterizations of pichinde viral nucleoprotein exoribonuclease functions.
UNLABELLED Arenaviruses cause severe hemorrhagic fever diseases in humans, and there are limited preventative and therapeutic measures against these diseases. Previous structural and functional analyses of arenavirus nucleoproteins (NPs) revealed a conserved DEDDH exoribonuclease (RNase) domain that is important for type I interferon (IFN) suppression, but the biological roles of the NP RNase i...
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We identified an error in our review “Animal Models for the Study of Rodent-Borne Hemorrhagic Fever Viruses: Arenaviruses and Hantaviruses” [1]. In Section 2.6 “Development of Low Containment Arenavirus Animal Models through Adaptation of Pichinde Virus to Hamsters and Guinea Pigs” the sentence, “This may be owed to similar receptor usage as NW arenavirus of clade A usurp α-dystroglycan for cel...
متن کاملSequencing studies of pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA.
Analyses of the complete sequence of the 1.1 X 10(6)-dalton, small (S) RNA of the arenavirus Pichinde and virus-induced cellular RNA species have revealed that the viral nucleoprotein, N, is coded in a subgenomic, non-polyadenylated, virus-complementary mRNA corresponding to the 3' half of the viral RNA (Auperin et al., Virology 134:208-219, 1984). By contrast, a second S-coded product, presuma...
متن کاملAlterations in NF- B and RBP-J by Arenavirus Infection of Macrophages In Vitro and In Vivo
Pichinde virus is an arenavirus that infects guinea pigs and serves as an animal model for human Lassa fever. An attenuated Pichinde virus variant (P2) and a virulent variant (P18) are being used to delineate pathogenic mechanisms that culminate in shock. In guinea pigs, the infection has been shown to begin in peritoneal macrophages following intraperitoneal inoculation and then spreads to the...
متن کاملPolymerase activity of Pichinde virus.
Pichinde virus, a member of the arenavirus group, was examined for polymerase activity. Purified virus was found to contain RNA-dependent RNA polymerase but not RNA-dependent DNA polymerase activity. Since RNase but neither DNase nor actinomycin D inhibited the endogenous polymerase reaction, RNA of the virus appeared to be used as the template. The divalent cations Mg(2+) and Mn(2+) were requi...
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عنوان ژورنال:
- Journal of virology
دوره 24 2 شماره
صفحات -
تاریخ انتشار 1977